锌指
MAPK/ERK通路
免疫沉淀
生物
支架蛋白
信号转导
细胞生物学
细胞质
互补DNA
分子生物学
激酶
抑制因子
基因
转录因子
生物化学
作者
Zhiming Xiang,Wuzhou Yuan,Na Luo,Yuequn Wang,Kai Tan,Yun Deng,Xijin Zhou,Chuangbing Zhu,Yanmei Li,Mingyao Liu,Xiushan Wu,Yongqing Li
标识
DOI:10.1016/j.bbrc.2006.06.076
摘要
Mitogen-activated protein kinases (MAPKs) are evolutionarily conserved enzymes in cell signal transduction. Previous studies revealed that zinc finger proteins are involved in the regulation of the MAPK signaling pathways. Here we report the identification and characterization of a novel human zinc finger protein, ZNF540. The cDNA of ZNF540 is 3.3kb, encoding 660 amino acids in the nucleus and the cytoplasm. Northern blot analysis indicates that ZNF540 is expressed in most of the fetal tissues. Overexpression of FLAG-ZNF540 in COS-7 cells represses the transcriptional activities of SRE and ELK-1, which can be relieved by siRNA. MVP, one of MAPK scaffold proteins, is identified as a potential ZNF540-binding protein. This interaction is detected by a yeast two-hybrid assay, reporter gene assays, and co-immunoprecipitation. Taken together, these results suggest that ZNF540 may act as a transcriptional repressor in MAPK signaling pathway to mediate cellular functions.
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