生物合成
萜类
代谢途径
生物
生物化学
甲戊酸途径
莽草酸途径
二萜
激发子
新陈代谢
酶
作者
Stefan Bartram,Andreas Jux,Gerd Gleixner,Wilhelm Boland
出处
期刊:Phytochemistry
[Elsevier]
日期:2006-08-01
卷期号:67 (15): 1661-1672
被引量:103
标识
DOI:10.1016/j.phytochem.2006.02.004
摘要
Two independent pathways contribute in higher plants to the formation of isopenteny1 diphosphate (IDP), the central building block of isoprenoids. In general, the cytosolic mevalonate pathway (MVA) provides the precursors for sesquiterpenes and sterols, whereas the plastidial methylerythritol pathway (MEP) furnishes the monoterpene-, diterpene- and carotenoids. Administration of deuterium labeled 1-deoxy-d-xylulose and mevalolactone to lima beans (Phaseolus lunatus), followed by gas chromatographic separation and mass spectrometric analysis of de novo produced volatiles revealed that the strict separation of both pathways does not exist. This could be confirmed by blocking the pathways individually with cerivastatin® (MVA) and fosmidomycin (MEP), respectively. Isotopic ratio mass spectrometry (IRMS) at natural abundance levels demonstrated independently and without the need for labeled precursors a dynamic allocation of the MVA- or the MEP-pathway in the biosynthesis of the nerolidol-derived homoterpene 4,8-dimethy1-nona-1,3,7-triene (DMNT). Insect-feeding upregulated predominantly the MVA-pathway, while the fungal elicitor alamethicin stimulated the biosynthesis of DMNT via the MEP-pathway.
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