介孔二氧化硅
药物输送
纳米颗粒
乙二醇
PEG比率
阿霉素
内吞作用
化学
MTT法
纳米技术
介孔材料
生物物理学
核化学
化学工程
材料科学
有机化学
细胞
生物化学
外科
催化作用
经济
工程类
生物
化疗
医学
财务
作者
Jinlou Gu,Shasha Su,Mingjie Zhu,Yongsheng Li,Wenru Zhao,Yourong Duan,Jianlin Shi
标识
DOI:10.1016/j.micromeso.2012.05.035
摘要
A layer of PEG (poly(ethylene glycol))-galactose was successfully grafted onto the external surface of mesoporous silica nanoparticles (MSNs) via a new silane-free approach, while the internal surface of MSNs was preserved for the encapsulation of the widely used anti-cancer drug of doxorubicin (DOX). The nanosized morphology and ordered structure of the synthesized drug delivery vehicles were verified by XRD and TEM observations. The successful grafting of PEG layer and peripherally exposed galactose ligands on the external surface of MSNs (abbreviated as MSNs-P/G) was confirmed by FT-IR and solid 13C NMR. The high-density PEG layer effectively reduced the human serum protein (HSP) adsorbance to the surface of MSNs. The maximum DOX loading amount reached as high as 900 mg/g and the loaded drug released in a pH-dependent way. Both confocal laser scanning microscopy (CLSM) observation and flow cytometry measurements supported the facts that cellular uptake of MSNs-P/G was significantly higher than that of the pristine MSNs benefitting from the galactose-receptor-mediated endocytosis process. This was consistent with the higher cytotoxicity observed with the [email protected]/G against the HepG2 cell line by MTT measurements.
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