细胞毒性
溶血
体外
化学
细胞凋亡
细胞毒性T细胞
抗菌剂
真皮成纤维细胞
细胞培养
成纤维细胞
微生物学
生物化学
生物
免疫学
遗传学
作者
César Burgos‐Díaz,Raquel Martín‐Venegas,Verónica Martı́nez,Carolina E Storniolo,José A. Teruel,Francisco J. Aranda,Antonio Gómez Ortíz,Ángeles Manresa,Ruth Ferrer,Ana Marqués
标识
DOI:10.1016/j.ijpharm.2013.06.029
摘要
The application of biosurfactants in the biomedical field is growing due to their antimicrobial activity, low cytotoxicity and ability to induce apoptosis in cancer cells. In the light of this therapeutic potential, as well as possible applications in cosmetics or as drug vehicles in pharmaceutical products, a new biosurfactant produced by Sphingobacterium detergens was investigated for its haemolytic activity and cytotoxic and antiproliferative effects in different cell lines. Fraction A showed 100% haemolysis in rabbit erythrocytes, but in Fraction B the rate was only 83%. When comparing cytotoxicity values (IC50) of the two fractions in model fibroblast and keratinocyte cell cultures, Fraction B was less cytotoxic, showing lower values than the reference compound SDS, indicating low skin irritability. Finally, in non-differentiated intestinal Caco-2 cultures, Fractions A and B reduced cell proliferation and induced apoptosis by 44% and 75%, respectively. According to these results, biosurfactants produced by S. detergens have potential application in cosmetic and pharmaceutical formulations.
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