细胞间粘附分子-1
ICAM-1
粘附
拉伤
细胞生物学
内皮干细胞
细胞内
细胞粘附分子
细胞粘附
内皮
化学
分子生物学
生物
生物化学
体外
解剖
内分泌学
有机化学
作者
Jing-Jy Cheng,Being-Sun Wung,Yeun-Jen Chao,Danny J.J. Wang
出处
期刊:Hypertension
[Ovid Technologies (Wolters Kluwer)]
日期:1996-09-01
卷期号:28 (3): 386-391
被引量:88
标识
DOI:10.1161/01.hyp.28.3.386
摘要
Since endothelial cells are constantly subjected to pressure-induced strain, we examined how cyclic strain affects the expression of intercellular adhesion molecule-1 (ICAM-1). Endothelial cells grown on a flexible membrane base were deformed by different sinusoidal negative pressures (-10, -15, or -20 kPa) to produce an average strain of 9%, 11%, and 12%, respectively, for various times. The release of the soluble form of ICAM-1 from strained endothelial cells increased in a time- and strain-dependent manner. Using flow cytometric analysis, we showed the induction of ICAM-1 expression on the endothelial cell surface to depend on both time and the amount of strain. Northern blot analysis revealed a sustained, approximately 1.8-fold increase in ICAM-1 mRNA levels in 11% strained cells. Strain-induced expression of ICAM-1 correlated with a strain-dependent increase in adhesion of monocytic cells to strained cells. This increase in monocytic cell adhesion could be partially inhibited by pretreatment of strained cells with antibody to ICAM-1. These results indicate that mechanical strain can stimulate the expression of ICAM-1 by endothelial cells and thus contribute to the increased adhesion of monocytes to strained cells. Such strain-induced expression of ICAM-1 may contribute to the trapping of monocytes on local vascular walls where strain is high and to the initiation of atherogenesis, thus providing a possible link between hypertension and atherogenesis.
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