Penetrate-Then-Gate Bioorthogonal In Situ Cancer Vaccination for Aligned Antigen Capture and Localized TLR7/8 Licensing

before irradiation), aligning exposure with the licensing window. Across multiple cancer models, coadministration improved primary control, elicited rechallenge protection, and suppressed distant growth, with restrained systemic cytokines. This work applies a spatiotemporal alignment principle for in situ vaccination to achieve precise and durable tumor immunotherapy in a modular way.