The predominant strain of Flammulina velutipes in liquid culture was screened by testing mycelial diameter,biomass(mycelium dry weight,md)and density.The results of antagonistic test and esterase isozymes analysis showed that the six varieties of Flammulina velutipes were different.The experiment indicated that the highest biomass was Japan PlatinumFlammulina velutipes and Hebei Flammulina velutipes,which were 1.628g/100mL and 1.662g/100mL,and the mycelia diameter were 0.203cm and 0.233cm,the mycelial number were 3 045/100 mL and 2 631/100 mL.Furthermore,in these two varieties of Flammulina velutipes Japan PlatinumFlammulina velutipes grew slow,which was 0.349cm/d and Hebei Flammulina velutipes were 0.460cm/d.Based on the results,it was founded that Hebei Flammulina velutipes was the predominant strains of Flammulina velutipes reduced liquid strain.