Flower Morphogenesis and Microspore Development Versus Anther Culture of Cucumber

Microspore development showed correlativity with the morphological characteristics of buds,and the color of anthers.The experiment was intended to determine the stage of anther development at which anthers could be optimally cultured,and the corresponding standards that could be adopted to select buds.The major factors affecting anther culture such as genotypes and pretreatment were studied,shich indicated that the buds differed in length with different genotypes at middle to later monokaryon stages of microspore development but did not differed in morphology with different genotypes;flower characteristies and anther inductivities of same genotypes differed at different stage of microspore development and the highest anther inductivity appeared at middle to late monokaryon stages.The pretreatment with low temperature was beneficial to maintaining microspore vitality.For anther culture,the pretreatment in dark with low temperature at 4℃ for 48～72 h was the most conducive to callus occurrence.The experimental results demonstrated that in the culture of cucumber anthers,the microspores could be optimally cultured at the middle to late monokaryon stages of microspore development and the bud-selecting standards were that the buds were 0.90～1.5 cm long and green,the crowns were not open in their top ends,and the anthers were white green or light green.