in vitro Rapid Propagation of GF677 Peach Rootstock

The technology of in vitro rapid propagation of GF677 peach rootstock was studied using new shoots and stem sections with axillary bud as explants.There were 6 kinds of basal medium(MS,WPM,G,RO,Modified DKW,Modified QL) with different ration hormones.The results showed that the good materials for the primary culture came from the hydroponics.The polluted rate was lower than twenty percent.The optimal basal medium for the primary culture and subculture and induction root was modified QL.It include QL(macro-element)+MS(micro-element)+DKW(FeNa2EDTA)+RO(Organic content).Modified QL supplemented with 1.0 mg·L-1 6-BA+0.01 mg·L-1 NAA+0.1 mg·L-1 GA3 was the most suitable for the fascicular buds induced with 3~8 buds.At the same time,modified QL supplemented with 0.8 mg·L-1 6-BA+0.01 mg·L-1 NAA was the most suitable for the adventitious shoots multiplication and the propagation rate reached 4~7 times.Half-strength MS liquid media supplemented with 1.2 mg·L-1 6-BA for 10 days,length of shoots were notably elongated.Also,modified QL supplemented with 1.0 mg·L-1 IBA+0.05 mg·L-1 NAA+80 mg·L-1 PG+50 mg·L-1 AC was the most suitable medium for elongated shoot rooting with 96.7% of rooting rate and 0.4 cm of average root length after 10 days.After the plantlets were transplanted into the greenhouse,the temperature(26±2)℃ and relative humidity(95%) must be carefully controlled in the first week.The new rooting and new leaf occurred in the greenhouse after 2~3 weeks.The survival rate was up to 85%.