Determiantion of Peroxynitrite by Flow Injection Chemiluminescence

Based on the chemiluminescence intensity of the reaction of Na2SO3-ONOO-fluorescein was linearly relationship with the concentration of peroxynitrite,a novel flow injection chemiluminescence(FICL) method for the determination of peroxynitrite has been developed.Under optimized conditions,linear range of this method was 2.384×10-6—1.192×10-4mol/L with r2 0.9994,the lowest concentration that can be detected was 2.238×10-6mol/L and the relative standard deviation(RSD) was 0.36%.This method was compared with luminol system,which obtained satisfactory result.