长双歧杆菌
生物
亚种
多位点序列分型
扩增片段长度多态性
基因型
遗传学
微生物学
遗传多样性
双歧杆菌
细菌
人口
动物
基因
人口学
社会学
乳酸菌
作者
Emiko Yanokura,Kaihei Oki,Hiroshi Makino,Monica Marianna Modesto,Bruno Pot,Paola Mattarelli,Bruno Biavati,Koichi Watanabe
标识
DOI:10.1016/j.syapm.2015.05.001
摘要
The species Bifidobacterium longum is currently divided into three subspecies, B. longum subsp. longum, B. longum subsp. infantis and B. longum subsp. suis. This classification was based on an assessment of accumulated information on the species' phenotypic and genotypic features. The three subspecies of B. longum were investigated using genotypic identification [amplified-fragment length polymorphism (AFLP), multilocus sequence analysis (MLSA) and multilocus sequence typing (MLST)]. By using the AFLP and the MLSA methods, we allocated 25 strains of B. longum into three major clusters corresponding to the three subspecies; the cluster comprising the strains of B. longum subsp. suis was further divided into two subclusters differentiable by the ability to produce urease. By using the MLST method, the 25 strains of B. longum were divided into eight groups: four major groups corresponding to the results obtained by AFLP and MLSA, plus four minor disparate groups. The results of AFLP, MLSA and MLST analyses were consistent and revealed a novel subspeciation of B. longum, which comprised three known subspecies and a novel subspecies of urease-negative B. longum, for which the name B. longum subsp. suillum subsp. nov. is proposed, with type strain Su 851(T)=DSM 28597(T)=JCM 19995(T).
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