Tissue-engineered Epithelium Transplantation for Severe Ocular Surface Burns

Purpose:To evaluate the clinical outcomes of tissue -engineered epithelium transplantation for severe ocular surface burns. Methods:This was a retrospective observational case series. From October 2005 to May 2011, 19 eyes of 19 patients with grade IV to VI ocular surface burns (Dua Classification) were treated by autologous transplantation of corneal stem cells cultivated on a fibrin gel membrane,with a mean follow-up of 16.2 months (range 12-36 months). Postoperative corneal surface stability, visual acuity (VA), corneal opacity, and neo- vascularization were evaluated. Results:No corneal perforations occurred and the entire corneal surface was free from epithelial defects in all eyes. At the final follow-up visit,VA in 17 eyes was improved after surgery,with 6 eyes achieving a VA of 20 / 100 or better. Corneal vascularization was significantly reduced in 17 (89.5% ).eyes.Corneal opacity was also improved in 12 (63.2%) eyes. All donor eyes remained healthy. Conclusion:Tissue-engineered epithelium transplantation can promote rapid reepithelialization of the ocular surface, inhibit corneal neovascularization,and improve vision for patients with severe ocular surface burns.