3D-printed autonomous microfluidics: Bioassay development towards the point of care

Here, we present a radically novel method for fabricating 3D passive microfluidics in one piece that is capable of accommodating multistep bioassays and actuating the fluids within the channels without any external devices.Furthermore, this 3D printed (3DPed) platform allows the incorporation of semi-quantitative readout, next to being easily scalable and cost-effective.As such, it represents a very attractive concept for point-of-care (POC) testing in low resource settings that comply with the WHO guidelines to be affordable, sensitive, specific, user-friendly, rapid, equipment-free and delivered to those who need it (ASSURED) 1 .