Changes in intestinal flora in preeclampsia rats and effects of probiotics on their inflammation and blood pressure.

OBJECTIVE The aim of this study was to investigate the changes in intestinal flora in preeclampsia rats and the effects of probiotics on their inflammation and blood pressure. MATERIALS AND METHODS A total of 40 Specific Pathogen Free (SPF) Wistar rats were randomly selected in this study. Abdominal operation was performed to reduce uterine blood perfusion, so as to establish the model of preeclampsia in rats. All rats were randomly divided into two groups, namely, observation group (treated with probiotics, n=20) and control group (not treated with probiotics, n=20). Subsequently, the changes in serum endotoxin level during intervention, the 24-h urinary 99mTc-diethylene triamine pentaacetic acid (DTPA) excretion rate, and intestinal flora colonization ability after intervention were compared between the two groups. The distribution of intestinal flora after intervention was recorded in the two groups. Meanwhile, vascular endothelial function and blood pressure following intervention were compared between the two groups as well. In addition, the changing trend of inflammatory cytokines during intervention in the two groups, and the correlation of colonization ability of intestinal flora with changes in systolic blood pressure and high-sensitivity C-reactive protein (hs-CRP) level in patients were analyzed. RESULTS At 3 days and 1 week after intervention, serum endotoxin level in the observation group was significantly lower than that in the control group at the same period (p<0.05). Following intervention, observation group exhibited remarkably higher excretion rate of 24-h urinary 99mTc-DTPA (p<0.05), stronger colonization ability of intestinal flora (p<0.05), higher levels of Bifidobacteria and Lactobacillus in intestinal flora (p<0.05), lower level of endothelin-1 (ET-1) (p<0.05) and higher level of nitric oxide (NO) (p<0.05) than the control group. In addition, the systolic blood pressure and diastolic blood pressure in the observation group were basically normal, which were both notably lower than those in the control group (p<0.05). At 3 days and 1 week after intervention, the levels of serum inflammatory cytokine hs-CRP in the observation group was markedly lower than that in the control group (p<0.05) at the same period. Furthermore, the colonization ability of intestinal flora was negatively associated with the changes in systolic blood pressure and hs-CRP level in patients (p<0.05). CONCLUSIONS Treating preeclampsia rats with probiotics can effectively reduce the level of serum endotoxin, improve the body's capacity to eliminate metabolites and the colonization ability of intestinal flora, maintain the stability of intestinal flora, enhance vascular endothelial function, and reduce blood pressure and the body's inflammatory responses.