基因敲除
上皮-间质转换
癌症研究
生物
肿瘤进展
污渍
免疫组织化学
转移
细胞
细胞迁移
细胞培养
基因
癌症
免疫学
遗传学
生物化学
作者
Jiajia Gao,Lusong Tian,Yulin Sun,Wei Li,Lina Zhao,Yan Sun,Zongpan Jing,Lanping Zhou,Fang Liu,Xiaohang Zhao
标识
DOI:10.1016/j.canlet.2020.10.030
摘要
Esophageal squamous cell carcinoma (ESCC) is one of the most common lethal cancers in the world. Dysregulation of purine-rich element binding protein alpha (PURα), which contributes to the initiation of PURΑ syndrome, is reportedly involved in the progression of multiple cancers, but its function and underlying mechanisms in ESCC progression remain unclear. Here, we first demonstrated that PURα promoted cell growth, migration and invasion in ESCC both in vitro and in vivo. An immunohistochemistry assay was then performed on 225 ESCC tissues, showing that high PURα expression was positively associated with lymph node metastasis and the AJCC stage, and the ESCC patients with positive PURα expression had worse survival. In addition, RNA sequencing implied that PURα induced epithelial-mesenchymal transition (EMT) in ESCC, which was further confirmed by qPCR, Western blotting and immunofluorescence analyses. Mechanistically, PURα enhanced the transcription of Snail2 by binding to its promoter region. Knockdown of Snail2 reversed PURα-induced EMT and inhibited the migration and invasion of ESCC cells. In conclusion, this study indicated that PURα promotes Snail2 transcriptional activity to induce EMT during ESCC progression.
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