植物化学
表皮葡萄球菌
抗菌剂
金黄色葡萄球菌
抗菌活性
奇异变形杆菌
琼脂扩散试验
寻常变形杆菌
枯草芽孢杆菌
化学
传统医学
大肠杆菌
微生物学
生物
细菌
生物化学
医学
基因
遗传学
作者
Sajid Bashir,Rubina Naureen Sial,Muhammad Ashraf,Muhammad Naveed Mushtaq,Muhammad Abdul-Jabar Adnan,Alia Erum
出处
期刊:PubMed
日期:2018-11-01
卷期号:31 (6): 2429-2434
摘要
In present investigation aqueous and methanolic extracts of Nelumbium speciosum flowers were screened for phytochemical constituents and antibacterial activity to ascertain their traditional use. Antimicrobial activity of both extracts was tested by Kirby-Bauer disc diffusion method against four Gram positive strains, viz. Staphylococcus aureus (ATCC25923), Streptococcus agalactiae (13813), Bacillus subtilis (ATCC 6633), Staphylococcus epidermidis (ATCC 12228) and four Gram negative bacterial strains Escherichia coli (ATCC 8739), Pseudomonas aeruginosa (ATCC 27853), Proteus mirabilis (12453) and Klebsiella pneumoniae (10031). Phytochemical analysis showed the presence of tannins, saponins and alkaloids in both extracts while flavonoids and steroids were present only in methanolic extract. Methanolic extract of Nelumbium speciosum flower showed concentration dependent antibacterial activity against all tested strains with maximum zone of inhibition (17.3±0.3mm) against P. aeruginosa. Aqueous extract showed concentration dependent activity against S. aureus, E. coli, B. subtilis and S. epidermidis with maximum antibacterial activity against E. coli (14.3±0.3mm). MIC of methanolic and aqueous extracts was in the range of 0.015-0.251 and 0.0625-0.251 mg/mL, respectively. Results showed that methanolic extract of Nelumbium speciosum exhibits superior antibacterial activity than aqueous extract.
科研通智能强力驱动
Strongly Powered by AbleSci AI