生物素化
化学
荧光
亲和素
定量分析(化学)
色谱法
生物素
血液蛋白质类
生物化学
生物物理学
量子力学
生物
物理
作者
Fang-Hong Lee,Chee Ying Chew,Jih Ru Hwu,Kui‐Thong Tan
标识
DOI:10.1002/jccs.201800293
摘要
The quantitative detection of biomarkers in protein‐rich samples using fluorescent probes is usually hampered by nonspecific fluorescence as a result of nonspecific probe–protein interactions. In this paper, we report a biotinylated fluorescent probe that is encapsulated in avidin protein, which can generate very specific fluorescence in blood serum by blocking out nonspecific dye–protein interactions. This approach was applied successfully to quantify glucose concentrations in blood serum.
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