过剩1
葡萄糖转运蛋白
生物
细胞周期
活力测定
糖酵解
细胞凋亡
葡萄糖转运蛋白1型
细胞内
细胞生长
癌细胞
细胞培养
葡萄糖摄取
分子生物学
细胞生物学
癌症研究
生物化学
癌症
酶
内分泌学
胰岛素
遗传学
作者
Yan Peng,Sining Xing,Hu-Ying Tang,Chang-dong Wang,Faping Yi,Geli Liu,Xiangbing Wu
出处
期刊:Gene
[Elsevier]
日期:2019-03-01
卷期号:689: 11-17
被引量:21
标识
DOI:10.1016/j.gene.2018.12.010
摘要
Most cancer cells predominantly produce their energy through a high rate of glycolysis in the presence of abundant oxygen. Glycolysis has become a target of anticancer strategies. Previous researches showed that glucose transporter 1 (GLUT1) inhibitor is effective as anticancer agents. This study assessed the effects of the selective GLUT1 inhibitor WZB117 on regulation of neuroblastoma (NB) cell line SH-SY5Y viability, cell cycle and glycolysis in vitro. SH-SY5Y cells were grown and treated with WZB117 for up to 72 h and then subjected to cell viability, qRT-PCR, Western blot and flow cytometry analysis. Level of ATP and LDH was also analyzed. The result showed that WZB117 treatment reduced tumor cells viability, downregulated level of GLUT1 protein. Moreover, WZB117 treatment arrested tumor cells at the G0-G1 phase of the cell cycle, induced tumor cells to undergo necrosis instead of apoptosis. In addition, WZB117 treatment downregulated the levels of intracellular ATP, LDH and glycolytic enzymes. Thus, WZB117-induced GLUT1 inhibition suppressed tumor cell growth, induced cell cycle arrest and reduced glycolysis metabolites in NB cells in vitro. This study suggested that GLUT1 can be used as a potential therapeutic target for NB.
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