循环肿瘤细胞
聚二甲基硅氧烷
上皮细胞粘附分子
生物医学工程
材料科学
癌细胞
模块化设计
癌症
纳米技术
计算机科学
医学
内科学
转移
操作系统
作者
He Sun,Lulu Han,Liwei Yang,Yan Yang,Wenning Jiang,Ting Xu,Lingyun Jia
出处
期刊:ACS Biomaterials Science & Engineering
[American Chemical Society]
日期:2019-03-29
卷期号:5 (5): 2647-2656
被引量:5
标识
DOI:10.1021/acsbiomaterials.8b01605
摘要
The capture of circulating tumor cells (CTCs) is mainly carried out with a small volume of blood using magnetic nanoparticles and complex microfluidics. In this study, we propose a CTC-capture apparatus based on a modular design and called this apparatus as the CTC chamber. Distinct from other CTC-capture apparatuses, the capacity of the CTC chamber could be altered by varying the number of CTC-capture modules to accommodate the different volumes of blood sample. The core component of the CTC-capture module was a polydimethylsiloxane (PDMS) film with cell-replicated topological structure and anti-EpCAM antibody coating. Both synergistic roles can enhance the capture yield of cancer cells. Furthermore, the CTC chamber was assembled with one or three CTC-capture modules for the capture of cancer cells from spiked blood samples representing late-stage (3 mL of blood, 10 cancer cells mL–1) or middle-early stage (9 mL of blood, 1 cancer cell mL–1) cancer. The results showed that high capture yield (EpCAM-positive, ∼80%; EpCAM-negative, ∼65%) and purity (EpCAM-positive, ∼90%; EpCAM-negative, ∼80%) could be obtained within 1 h. This economic and facile CTC chamber could therefore open up opportunities for designing the next-generation CTC detection devices suitable for the diagnosis of different stages of cancer.
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