分解代谢
尼古丁
羟基化
化学
假单胞菌
生物化学
拉伤
水解
生物碱
细菌
新陈代谢
生物
立体化学
酶
遗传学
解剖
神经科学
作者
Dandan Pan,Mengmeng Sun,Yawen Wang,Pei Lv,Xiangwei Wu,Qing X. Li,Haiqun Cao,Rimao Hua
标识
DOI:10.1021/acs.jafc.8b01868
摘要
Nicotine is a major toxic alkaloid in wastes generated from tobacco production and cigarette manufacturing. In the present work, a nicotine-degrading bacterial strain was isolated from tobacco powdery waste. The isolate was identified as Pseudomonas sp. S-1 based on morphology, physiology, and 16S rRNA gene sequence. Suitable conditions of isolate S-1 for nicotine degradation were pH 7.0 and 30 °C. Catabolic intermediates of nicotine were isolated with preparative-HPLC and characterized with LC-HRMS and NMR. The catabolic pathways of nicotine were involved in dehydrogenation, oxidation, hydrolysis, and hydroxylation. Interestingly, nicotine catabolism in strain S-1 undergoes a new pyrrolidine pathway that differs from the other three catabolic pathways in bacterial species. This work sheds light on catabolic diversity of nicotine and heteroaromatics.
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