色谱法
核酸
洗脱
化学
DNA
溶解
离心
离子色谱法
盐(化学)
离子交换
核糖核酸
裂解缓冲液
间隙
生物化学
离子
泌尿科
物理化学
有机化学
基因
医学
作者
Kim Budelier,Joachim Schorr
标识
DOI:10.1002/0471142727.mb0201bs42
摘要
Abstract Column chromatography has evolved to provide a rapid and effective alternative to more laborious methods for preparing high‐quality DNA, such as CsCl‐gradient centrifugation. This unit describes the use of a column made of a unique anion‐exchange resin that selectively binds nucleic acids, allowing rapid separation of DNA from contaminating RNA, proteins, carbohydrates, and metabolites. The procedure employs columns supplied by QIAGEN; other preparation methods are available from other suppliers. A crude nucleic acid sample (usually a cleared cell lysate) is applied to the QIAGEN tip under conditions that favor binding. Contaminants in the sample are washed from the column with a moderate‐salt buffer, and DNA is eluted using a high‐salt buffer.
科研通智能强力驱动
Strongly Powered by AbleSci AI