Histological and Histochemical Methods: Theory and Practice

This text presents the chemical and physical principles of fixation, staining and histochemistry. It assumes a basic level of chemical and biological knowledge, and requires little mathematical skill. The relations of chemical structures and reactions to fixation, tissue processing, staining, enzyme location, immunohistochemistry and other procedures are explained. For this edition the author has updated the text with the latest techniques and developments within the field, whilst retaining the details of the classic techniques still in use. The book offers a practical guide to the preparation of specimens for light microscopy and includes detailed practical instructions of the techniques used. This text presents the chemical and physical principles of fixation, staining and histochemistry. It assumes a basic level of chemical and biological knowledge, and requires little mathematical skill. The relations of chemical structures and reactions to fixation, tissue processing, staining, enzyme location, immunohistochemistry and other procedures are explained. For this edition the author has updated the text with the latest techniques and developments within the field, whilst retaining the details of the classic techniques still in use. The book offers a practical guide to the preparation of specimens for light microscopy and includes detailed practical instructions of the techniques used.