脂肪酸合成
脂肪酸
生物
脂质代谢
多不饱和脂肪酸
甾醇调节元件结合蛋白
哺乳期
生物化学
细胞生物学
化学
基因
基因表达
遗传学
怀孕
作者
Lian Huang,Jun Luo,Wenchang Gao,Ning Song,Hui Tian,Lu Zhu,Qianming Jiang,Juan J. Loor
出处
期刊:Foods
[MDPI AG]
日期:2022-07-07
卷期号:11 (14): 2012-2012
被引量:3
标识
DOI:10.3390/foods11142012
摘要
In nonruminants, microRNA (miRNA)-24 plays an important role in lipid metabolism in adipose tissue and the liver. Although the abundance of miR-24 in ruminant mammary glands is the highest during peak lactation, its potential role in regulating the synthesis and secretion of fat into milk is unclear. This study aimed to identify the function of miR-24 in these processes using CRISPR/Cas9 technology in primary goat mammary epithelial cells (GMEC). A single clone containing a 66-nucleotide deletion between two sgRNAs mediating double-strand break (DSB) sites was obtained. The abundance of miR-24-3p and miR-24-5p encoded by the deleted sequence was decreased, whereas the target genes INSIG1 and FASN increased. In addition, miR-24 knockout reduced the gene abundance of genes associated with fatty acid and TAG synthesis and transcription regulator. Similarly, the content of cholesterol and monounsaturated fatty acid (MUFA) C18:1 decreased, whereas that of polyunsaturated fatty acids (PUFA) C18:2, C20:3, C20:4 and C20:5 increased. Subsequently, knocking down of INSIG1 but not FASN reversed the effect of miR-24 knockout, indicating that miR-24 modulated cholesterol and fatty acid synthesis mainly by targeting INSIG1. Overall, the present in vitro data demonstrated a critical role for miR-24 in regulating lipid and fatty acid synthesis and highlighted the possibility of manipulating milk components in dairy goats.
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