细胞外小泡
小RNA
胞外囊泡
逆转录聚合酶链式反应
逆转录酶
微泡
实时聚合酶链反应
计算生物学
支气管肺泡灌洗
分子生物学
RNA提取
生物
化学
核糖核酸
细胞生物学
信使核糖核酸
基因
生物化学
医学
内科学
肺
作者
Ayyanar Sivanantham,Heedoo Lee,Yang Jin
标识
DOI:10.1007/978-1-0716-2341-1_10
摘要
Extracellular vesicles (EVs) are biological carriers, and EV-associated miRNAs (EV-miRNAs) are considered as a novel biomarker in multiple diseases. Currently, the column-based purification method is used to purify miRNAs from EVs. However, this method of purification is complex, time-consuming, and expensive. Therefore, a simple and cost-effective single-step quantitative reverse transcription-polymerase chain reaction (RT-qPCR) method is required to detect the expression of EV-miRNAs. This chapter describes a protocol for directly analyzing the EV-miRNAs expression from mouse bronchoalveolar lavage fluid (BALF) and serum without going for an RNA isolation and purification step from EVs. It is an efficient method in several terms such as cost-wise, time, low expertise, and accuracy in results. This method may be helpful in diagnostic blood tests used in medical centers or research laboratories.
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