氧气增强率
流式细胞术
线性能量转移
A549电池
辐照
DNA损伤
相对生物效应
氧气
辐射
癌细胞
分子生物学
辐射敏感性
细胞仪
化学
DNA
细胞
离子
生物物理学
癌症
生物
物理
生物化学
遗传学
有机化学
核物理学
量子力学
作者
Shigeaki Sunada,Hirokazu Hirakawa,A. Fujimori,Mitsuru Uesaka,Ryuichi Okayasu
出处
期刊:Radiation Research
[BioOne (Radiation Research Society)]
日期:2017-11-01
卷期号:188 (5): 671-674
被引量:9
摘要
High-linear energy transfer (LET) heavy ions cause higher therapeutic effects than low-LET radiation due to lower dependency on oxygen concentration in tumor cell killing. The lethality after irradiation largely depends on DNA double-strand breaks (DSBs), however the detailed LET dependency for DSB induction under oxic and hypoxic conditions has not been reported. Therefore, we evaluated the oxygen enhancement ratio (OER) of heavy ion-induced DSB induction using a highly-optimized flow cytometry-based method of γ-H2AX detection. Non-small cell lung cancer (NSCLC) A549 cells were exposed to X-ray, carbon-ion and iron-ion radiations under oxic or hypoxic condition. As a DSB marker, the γ-H2AX signal was measured 1 h postirradiation and analyzed by flow cytometry. DSB slope values were calculated as DSB induction per Gy. Our method was able to detect high-LET radiation-induced DSBs even from clustered DNA damage sites. We also showed a decrease in OER value in an LET-dependent manner regardless of radiation type. In summary, we demonstrated a simple, quick and highly-optimized flow cytometry-based method of DSB analysis that detects DSBs induced by heavy-ion radiation for hypoxic and nonhypoxic cancer cells. Our study may provide a useful biological basis for heavy-ion radiotherapy.
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