固有层
结肠炎
白细胞介素9
溃疡性结肠炎
免疫组织化学
炎症
抗体
细胞因子
病理
免疫学
上皮内淋巴细胞
CD3型
CD8型
白细胞介素
医学
生物
免疫系统
上皮
疾病
作者
Ang Yuan,Hang Yang,Haili Qi,Jing Cui,Hua Wei,Can Li,Zhigang Pang,Wei Zheng,Guanglin Cui
标识
DOI:10.1016/j.bbrc.2015.11.057
摘要
Diverse T help (Th) cells play a crucial role in the processing and maintaining of chronic inflammation as seen in ulcerative colitis (UC). Th9, a novel subset of Th cells that primarily produces interleukin (IL)-9, has recently been associated with the development of inflammatory diseases. In this study, we evaluated the presentation of Th9 cells in inflamed tissues of human and experimental mouse UC, and examined the therapeutic efficiency of anti Th9 cytokine IL-9 in the experimental mouse UC. Using immunohistochemistry (IHC), we evaluated the presentation of Th9 cells labelled by transcriptional factor PU.1 in both human and dextran sulfate sodium (DSS) induced mouse colitis biopsies. The results showed that increased PU.1 positive Th9 cells were mainly located in the lamina propria in relative with the controls, intraepithelial Th9 cells can also be observed but at low density. Double IHCs revealed that most of PU.1 positive cells were CD3 positive lymphocytes in human UC specimens. Anti-IL-9 antibody injection for 2 weeks reduced the severity of inflammation in DSS induced colitis mice. Our results suggest that The Th9/IL-9 is involved in the pathogenesis of UC.
科研通智能强力驱动
Strongly Powered by AbleSci AI