Invertase Production by S. cerevisiae MTCC 170 Using Statistical Tool Central Composite Design

Invertase producing yeast S. cerevisiaewas obtained from MTCC. The optimal level of the key variables (orange peel, yeast extract and methionine) used to determine the effect of their interactions on invertase production using the statistical tool (CCD of RSM). The second-order quadratic model with the optimum conditions (orange peel - 4%;yeast extract - 0.5% andmethionine - 0.5%). The nearness of the coefficient of determination (R =0.9948) to 1 ensures the satisfactory adjustment of the quadratic model to the 2 experimental data. The maximum invertase production was calculated as 0.37 IU/ml.