雷加诺森
化学
色谱法
蛋白质沉淀
药代动力学
醋酸铵
选择性反应监测
串联质谱法
质谱法
高效液相色谱法
液相色谱-质谱法
作者
Dun‐Jian Wang,Da‐Wei Wang,Qiu‐Chen Fang,Ye Shen,Nv‐Jin Zeng,Yan‐Ling Yang,Hong‐Wen Zhang,Yong-Qing Wang,Lu‐Ning Sun
标识
DOI:10.1002/jssc.202100756
摘要
Regadenoson, the first selective adenosine A2A receptor agonist, is used to perform exercise stress test during radionuclide myocardial perfusion imaging. To detect the concentration of regadenoson in human plasma, a simple, fast, and sensitive tandem mass spectrometry method was established herein. Acetonitrile was used as a protein precipitation agent. Chromatographic separation was completed in 6.5 min using a BEH HILIC column (50 × 2.1 mm, 1.7 μm). The mobile phase consisted of 10 mmol/L ammonium acetate/acetonitrile (gradient elution). To quantify regadenoson and regadenoson-d3, an API 4000 mass spectrometry in multiple reaction monitoring mode with transitions of 391.3→259.2 and 394.3→262.2, respectively, was utilized. The calibration curve was linear in the range of 0.100-50.0 μg/L, and the intrabatch and interbatch precisions were <9.7% and <13.0%, respectively, and the accuracy was 2.0-6.9%. There was no apparent matrix effect for regadenoson or regadenoson-d3. The developed method was used to study the pharmacokinetic characteristics of regadenoson in healthy Chinese subjects.
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