神经保护
缺氧(环境)
MAPK/ERK通路
蛋白激酶B
药理学
细胞凋亡
海马结构
转录组
活力测定
绞股蓝
化学
信号转导
生物
神经科学
生物化学
基因表达
色谱法
萃取(化学)
基因
有机化学
氧气
作者
Yanan Geng,Ming Zhao,Jun‐Li Yang,Xiang Cheng,Ying Han,Chengbo Wang,Xiufang Jiang,Ming Fan,Ling‐Ling Zhu
标识
DOI:10.1016/j.taap.2022.116092
摘要
Gypenosides are major bioactive ingredients of G. pentaphyllum. In our previous study, we found that gypenosides had neuroprotective effects against hypoxia-induced injury. In the current study, we focused on the protective effects of gypenoside-14 (GP-14), which is one of the newly identified bioactive components, on neuronal injury caused by severe hypoxia (0.3% O2). The results showed that GP-14 pretreatment alleviated the cell viability damage and apoptosis induced by hypoxia in PC12 cells. Moreover, GP-14 pretreatment also attenuated primary neuron injuries under hypoxic conditions. Additionally, GP-14 pretreatment significantly ameliorated neuronal damage in the hippocampal region induced by high-altitude cerebral edema (HACE). At the molecular level, GP-14 pretreatment reversed the decreased activities of the AKT and ERK signaling pathways caused by hypoxia in PC12 cells and primary neurons. To comprehensively explore the possible mechanisms, transcriptome sequencing was conducted, and these results indicated that GP-14 could alter the transcriptional profiles of primary neuron. Taken together, our results suggest that GP-14 acts as a neuroprotective agent to protect against neuronal damage induced by severe hypoxia and it is a promising compound for the development of neuroprotective drugs.
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