生发中心
CD40
CD38
B细胞
细胞生物学
生物
幼稚B细胞
免疫球蛋白D
分子生物学
细胞培养
B-1电池
滤泡树突状细胞
CD44细胞
T细胞
化学
细胞
抗原提呈细胞
免疫学
细胞毒性T细胞
免疫系统
抗体
体外
干细胞
川地34
生物化学
遗传学
作者
Han‐Soo Kim,Xinhong Zhang,Elena N. Klyushnenkova,Yong Sung Choi
出处
期刊:Journal of Immunology
[The American Association of Immunologists]
日期:1995-08-01
卷期号:155 (3): 1101-1109
被引量:66
标识
DOI:10.4049/jimmunol.155.3.1101
摘要
Abstract To identify the signals given to the germinal center (GC) B cells by FDC, we have established an FDC-like cell line, HK. HK cells have the important functions of FDC, which are the preferential binding and stimulation of GC B cells. Cell-cell interaction between IgD- B cells and HK cells resulted in the rescue of these cells from apoptosis, whereas the majority of unbound B cells or B cells cultured in the absence of HK cells underwent apoptosis. We investigated the effects of HK cells on the subpopulations of tonsillar B lymphocytes that are at different stages of maturation and differentiation. The subpopulations of tonsillar B cells were purified by panning and/or MACS according to the surface expression of IgD, CD38, and CD44. Although HK cells alone did not stimulate B cell proliferation, HK cells in the presence of either anti-mu or anti-CD40 significantly enhanced the proliferation of B cells. The data show that HK cells preferentially bind, rescue, and stimulate GC B cells (IgD-CD38+) generating CD38-CD44+ memory B cells. The costimulation activity of HK cells is also provided in a form of soluble factor(s). Hence, the phenotypic and functional analysis of HK cells suggests that these cells may be derived from FDC.
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