生物
免疫监视
免疫系统
抗原
乳腺癌
B细胞
单细胞分析
细胞
癌症研究
单细胞测序
癌症
免疫学
基因
抗体
遗传学
表型
外显子组测序
作者
Qingxi Hu,Hong Yu,Qi Pan,Guangqing Lu,Xueying Mai,Sheng Xu,Xiaoying He,Yu Guo,Linlin Gao,Zhiyi Jing,Jiawen Wang,Tao Cai,Yu Zhang
标识
DOI:10.1038/s41467-021-22300-2
摘要
Abstract To gain mechanistic insights into the functions and developmental dynamics of tumor-infiltrated immune cells, especially B-lymphocytes, here we combine single-cell RNA-sequencing and antigen receptor lineage analysis to characterize a large number of triple-negative breast cancer infiltrated immune cells and report a comprehensive atlas of tumor-infiltrated B-lymphocytes. The single-cell transcriptional profiles reveal significant heterogeneity in tumor-infiltrated B-cell subgroups. The single-cell antigen receptor analyses demonstrate that compared with those in peripheral blood, tumor-infiltrated B-cells have more mature and memory B-cell characteristics, higher clonality, more class switching recombination and somatic hypermutations. Combined analyses suggest local differentiation of infiltrated memory B-cells within breast tumors. The B-cell signatures based on the single-cell RNA-sequencing results are significantly associated with improved survival in breast tumor patients. Functional analyses of tumor-infiltrated B-cell populations suggest that mechanistically, B-cell subgroups may contribute to immunosurveillance through various pathways. Further dissection of tumor-infiltrated B-cell populations will provide valuable clues for tumor immunotherapy.
科研通智能强力驱动
Strongly Powered by AbleSci AI