Comparison of Three Immunoassay Systems for Determining Serum Anti-Mullerian Hormone

Background Serum anti-Mullerian hormone (AMH) is a dimeric glycoprotein of the transforming growth factor-β (TGF-β) family of growth and serves as a key biomarker of folliculogenesis as well as steroidogenesis within ovaries. Cobas E601, UniCel DxI 800 and iFlash3000 are automated immunoassay systems which are widely used to measure serum AMH levels in the blood. However, the correlations of serum AMH measured by the three automated immunoassay systems are not well recognized, the aim of this study is to compare the performance of the three automated immunoassay systems and record the correlation of serum AMH concentrations. Methods Serum AMH concentrations were measured in 100 serum samples using the UniCel DxI 800, Cobas E601, and iFlash 3000 automated immunoassay systems on the same day. Passing-Bablok regression analysis and Bland-Altman plot analysis were used to compare the system methods. The concordance correlation coefficient was analyzed to explain interrater agreement between the three immunoassay systems. Results Bland-Altman plot showed that the concentrations of AMH measured by UniCel DxI 800 were about 0.15 times higher than that measured using Cobas E601, the concentrations of AMH measured by UniCel DxI 800 were about 0.05 times higher than that measured using iFlash 3000, serum AMH concentrations measured by the iFlash3000 were about 0.19 times higher than that measured using Cobas E601. The concordance correlation coefficient ρc was 0.921, 0.909 and 0.978 for the UniCel DxI 800 versus the Cobas E601, the iFlash 3000 versus the UniCel DxI 800, and the iFlash3000 versus the Cobas E601, respectively. Conclusions The three measurement systems have good correlation with each other for determining serum AMH.