Trapping of Proteins under Physiological Conditions in a Nanopipette

Caught in a trap and on camera: A nanopipette is used for electrodeless dielectrophoresis and clear evidence is shown, by using wide-field fluorescence imaging (see image), for the reversible trapping of Alexa-488-labeled proteins (protein G and immunoglobulin G) and also of the fluorophore alone. The dielectrophoretic concentration is enhanced by at least a factor of 300 for these fluorophore-labeled proteins. Supporting Information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2005/z500196_s.html or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.