生物
高尔基体
细胞生物学
泛素连接酶
秀丽隐杆线虫
生物化学
泛素
内质网
基因
作者
Albert Zhang,Ziqiang Guan,Kyle Ockerman,Dong Pengyuan,Hao Jia,Zhiping Wang,Dong Yan
出处
期刊:PLOS Biology
[Public Library of Science]
日期:2020-12-28
卷期号:18 (12): e3001051-e3001051
被引量:4
标识
DOI:10.1371/journal.pbio.3001051
摘要
Coordination of cell growth is essential for the development of the brain, but the molecular mechanisms underlying the regulation of glial and neuronal size are poorly understood. To investigate the mechanisms involved in glial size regulation, we used Caenorhabditis elegans amphid sheath (AMsh) glia as a model and show that a conserved cis -Golgi membrane protein eas-1/GOLT1B negatively regulates glial growth. We found that eas-1 inhibits a conserved E3 ubiquitin ligase rnf-145 /RNF145, which, in turn, promotes nuclear activation of sbp-1/ SREBP, a key regulator of sterol and fatty acid synthesis, to restrict cell growth. At early developmental stages, rnf-145 in the cis -Golgi network inhibits sbp-1 activation to promote the growth of glia, and when animals reach the adult stage, this inhibition is released through an eas-1- dependent shuttling of rnf-145 from the cis -Golgi to the trans -Golgi network to stop glial growth. Furthermore, we identified long-chain polyunsaturated fatty acids (LC-PUFAs), especially eicosapentaenoic acid (EPA), as downstream products of the eas-1-rnf-145-sbp-1 pathway that functions to prevent the overgrowth of glia. Together, our findings reveal a novel and potentially conserved mechanism underlying glial size control.
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