电化学
氧化还原
细菌
枯草芽孢杆菌
大肠杆菌
化学
电子转移
多物理
微生物
细菌细胞结构
生物物理学
生物化学
无机化学
电极
生物
光化学
物理化学
物理
热力学
有限元法
基因
遗传学
作者
Yafei Chen,Dengchao Wang,Yanran Liu,Guanyue Gao,Jinfang Zhi
标识
DOI:10.1016/j.bios.2020.112914
摘要
This paper reports on an innovative strategy based on the electrochemical collision technique to quantify the redox activity of two bacterial species: the Gram-negative Escherichia coli and the Gram-positive Bacillus subtilis. Thionine (TH), as a redox mediator, was electrostatically adsorbed on bacterial surface and formed the bacterium-TH complexes. TH can receive electrons from bacterial metabolic pathways and be reduced. When a single bacterium-TH complex collides on the ultramicroelectrode, the reduced TH will be re-oxidized at certain potential and generate current spike. The frequency of the spikes is linearly proportional to the living bacteria concentration, and the redox activity of individual bacterium can be quantified by the charges enclosed in the current spike. The redox ability of Gram-negative E.coli to the TH mediator was 6.79 ± 0.26 × 10−18 mol per bacterial cell in 30 min, which is relatively more reactive than B. subtilis (3.52 ± 0.31 × 10−18 mol per cell). The spike signals, fitted by 3D COMSOL Multiphysics simulation, revealed that there is inherent redox ability difference of two bacterial strains besides the difference in bacterial size and collision position. This work successfully quantified the bacterial redox activity to mediator in single cells level, which is of great significance to improve understanding of heterogeneous electron transfer process and build foundations to the microorganism selection in the design of microbial electrochemical devices.
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