体内
膜
阻隔膜
生物医学工程
再生(生物学)
苏木精
X射线显微断层摄影术
材料科学
牙科
化学
染色
病理
医学
细胞生物学
生物
生物化学
生物技术
放射科
作者
Seyed Javad Mousavi,Fatemeh Ejeian,Amir Razmjou,Mohammad Hossein Nasr‐Esfahani
摘要
Abstract Aim The profound potential of zeolitic imidazolate framework 8 (ZIF8) thin film for inducing osteogenesis has been previously established under in vitro conditions. As the next step towards the clinical application of ZIF8‐modified substrates in periodontology, this in vivo study aimed to evaluate the ability of the ZIF8 crystalline layer to induce bone regeneration in an animal model defect. Materials and Methods Following the mechanical characterization of the membranes and analysing the in vitro degradation of the ZIF8 layer, in vivo bone regeneration was evaluated in a critical‐sized (5‐mm) rat calvarial bone defect model. For each animal, one defect was randomly covered with either a polypropylene (PP) or a ZIF8‐modified membrane ( n = 7 per group), while the other defect was left untreated as a control. Eight weeks post surgery, bone formation was assessed by microcomputed tomography scanning, haematoxylin and eosin staining and immunohistochemical analysis. Results The ZIF8‐modified membrane outperformed the PP membrane in terms of mechanical properties and revealed a trace Zn +2 release. Results of in vivo evaluation verified the superior barrier function of the ZIF8‐coated membrane compared with pristine PP membrane. Compared with the limited marginal bone formation in the control and PP groups, the defect area was almost filled with mature bone in the ZIF8‐coated membrane group. Conclusions Our results support the effectiveness of the ZIF8‐coated membrane as a promising material for improving clinical outcomes of guided bone regeneration procedures, without using biological components.
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