信使核糖核酸
费斯特共振能量转移
DNA
生物物理学
核糖核酸
分子机器
DNA纳米技术
纳米技术
计算生物学
细胞内
级联
荧光
细胞生物学
生物
生物系统
化学
物理
材料科学
基因
遗传学
色谱法
量子力学
作者
Jinwen Zhu,Xiaolin Qu,Yuan Zhuang,Peng Miao
标识
DOI:10.1002/sstr.202300420
摘要
Messenger RNA (mRNA) is the molecule which carries genetic information from DNA and guides the synthesis of protein. It participates in many cellular cascades and exhibits substantial associations with certain diseases. The development of advanced approaches for mRNA sensing and imaging is of utmost importance for biological studies and early clinical diagnosis. Herein, a novel fluorescent method is demonstrated for highly sensitive analysis of mRNA based on entropy‐driven strand displacements around three‐dimensional DNA nanostructures. Tetrahedral DNA with four pendant linear sequences provides excellent molecular scaffold loading at multiple sites for target mRNA‐initiated cascade toehold strand displacement reactions. The variations of fluorescence resonance energy transfer states can be used to indicate the presence and level of target mRNA. Besides, MnO 2 nanosheets are introduced for intracellular transportation of the reaction system for bioimaging. The operation is relatively simple and the developed approach provides a promising tool for mRNA‐related biological researches and clinical applications.
科研通智能强力驱动
Strongly Powered by AbleSci AI