细胞外基质
卵母细胞
细胞生物学
自愈水凝胶
间质细胞
组织工程
基底膜
化学
细胞生长
细胞
层粘连蛋白
佩莱肯
生物物理学
材料科学
生物
生物化学
胚胎
蛋白多糖
癌症研究
遗传学
有机化学
作者
Claire E. Nason-Tomaszewski,Emily E. Thomas,Daniel L. Matera,Brendon M. Baker,Ariella Shikanov
标识
DOI:10.1016/j.bioactmat.2023.10.001
摘要
Synthetic matrices which mimic the extracellular composition of native tissue create a comprehensive model for studying development and disease. Here, we have engineered a composite material which retains cell-secreted ECM for the culture of ovarian follicles by embedding electrospun dextran fibers functionalized with basement membrane binder (BMB) peptide in PEG hydrogels. In the presence of ECM-sequestering fibers, encapsulated immature primordial follicles and ovarian stromal cells aggregated into large organoid-like structures with dense deposition of laminin, perlecan, and collagen I, leading to steroidogenesis and significantly greater rates of oocyte survival and growth. We determined that cell aggregation restored key cell-cell interactions critical for oocyte survival, whereas oocyte growth was dependent on cell-matrix interactions achieved in the presence of BMB. Here we have shown that sequestration and retention of cell-secreted ECM along synthetic fibers mimics fibrous ECM structure and restores the cell-cell and cell-matrix interactions critical for engineering an artificial ovary.
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