An Oxidative Enzyme Boosting Mechanical and Optical Performance of Densified Wood Films

Abstract Nature has evolved elegant ways to alter the wood cell wall structure through carbohydrate‐active enzymes, offering environmentally friendly solutions to tailor the microstructure of wood for high‐performance materials. In this work, the cell wall structure of delignified wood is modified under mild reaction conditions using an oxidative enzyme, lytic polysaccharide monooxygenase (LPMO). LPMO oxidation results in nanofibrillation of cellulose microfibril bundles inside the wood cell wall, allowing densification of delignified wood under ambient conditions and low pressure into transparent anisotropic films. The enzymatic nanofibrillation facilitates microfibril fusion and enhances the adhesion between the adjacent wood fiber cells during densification process, thereby significantly improving the mechanical performance of the films in both longitudinal and transverse directions. These results improve the understanding of LPMO‐induced microstructural changes in wood and offer an environmentally friendly alternative for harsh chemical treatments and energy‐intensive densification processes thus representing a significant advance in sustainable production of high‐performance wood‐derived materials.