结晶
成核
蛋白质结晶
生物制药
材料科学
化学
结晶学
化学物理
有机化学
生物
生物技术
作者
Hao Líu,Yue Zhao,Jing Sun
出处
期刊:Biomimetics
[MDPI AG]
日期:2023-02-06
卷期号:8 (1): 68-68
被引量:1
标识
DOI:10.3390/biomimetics8010068
摘要
Protein crystallization was first discovered in the nineteenth century and has been studied for nearly 200 years. Protein crystallization technology has recently been widely used in many fields, such as drug purification and protein structure analysis. The key to successful crystallization of proteins is the nucleation in the protein solution, which can be influenced by many factors, such as the precipitating agent, temperature, solution concentration, pH, etc., among which the role of the precipitating agent is extremely important. In this regard, we summarize the nucleation theory of protein crystallization, including classical nucleation theory, two-step nucleation theory, and heterogeneous nucleation theory. We focus on a variety of efficient heterogeneous nucleating agents and crystallization methods as well. The application of protein crystals in crystallography and biopharmaceutical fields is further discussed. Finally, the bottleneck of protein crystallization and the prospect of future technology development are reviewed.
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