平移(音频)
噬菌体展示
抗体
病毒学
穗蛋白
融合蛋白
冠状病毒
肽库
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
分子生物学
生物
抗原
免疫系统
2019年冠状病毒病(COVID-19)
基因
重组DNA
肽序列
传染病(医学专业)
医学
免疫学
疾病
生物化学
古生物学
病理
缩放
镜头(地质)
作者
Qianling Su,Wei Shi,Xuanping Huang,Yakun Wan,Guanghui Li,Bengang Xing,Zhi Ping Xu,Hongbo Liu,Bruce D. Hammock,Xiaomei Yang,Shutao Yin,Xiaoling Lü
出处
期刊:Cells
[MDPI AG]
日期:2022-10-24
卷期号:11 (21): 3355-3355
被引量:3
标识
DOI:10.3390/cells11213355
摘要
Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), an infectious disease that has become a serious burden on global public health. This study screened and yielded specific nanobodies (Nbs) against SARS-CoV-2 spike protein receptor binding domain (RBD), following testing its basic characteristics. A nanobody phage library was established by immunizing a camel with RBD protein. After three rounds of panning, the positive colonies were screened by enzyme-linked immunosorbent assay (ELISA). By sequencing, four different sequences of nanobody gene fragments were selected. The four nanobody fusion proteins were expressed and purified, respectively. The specificity and affinity of the four nanobodies were identified by ELISA. Our results showed that an immune phage display library against SARS-CoV-2 has been successfully constructed with a library capacity of which was 4.7 × 108 CFU. The four purified nanobodies showed specific high-affinity binding SARS-CoV-2 S-RBD. Among these, the antigen binding affinity of Nb61 was more comparable to that of commercial rabbit anti-SARS-CoV-2 S-RBD antibodies. In sum, our study has obtained four nanobody strains against SARS-CoV-2 S-RBD with significant affinity and specificity, therefore laying an essential foundation for further research as well as the applications of diagnostic and therapeutic tools of SARS-CoV-2.
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