多囊卵巢
生物
内分泌系统
衰老
异种雌激素
内分泌学
激素
内科学
雌激素
内分泌干扰物
糖尿病
医学
细胞生物学
雌激素受体
胰岛素抵抗
癌症
遗传学
乳腺癌
作者
Nimitha S. Prabhu,Divya Adiga,Shama Prasada Kabekkodu,Seetharama M Bhat,Kapaettu Satyamoorthy,S Padmalatha
标识
DOI:10.1016/j.etap.2022.104010
摘要
Bisphenol A (BPA) mimics estrogen and consequently suspected to be detrimental to female reproductive system. Biomonitoring confirms the BPA burden in body leading to a complex condition called polycystic ovarian syndrome (PCOS) which is frequently attributed to female infertility. Due to unclear precise molecular pathomechanisms of BPA in PCOS, we intend to examine the molecular mechanisms of the reproductive, endocrine, mitochondrial features, and cellular senescence in BPA-treated rats. We analyzed vaginal smears and ovarian follicles using microscope, assessed sex hormones by ELISA, analyzed BPA target gene expression by semi-quantitative RT-PCR, assessed senescence induction by β-galactosidase staining and immunofluorescence in BPA-treated rats. Our data showed hormonal imbalance, impaired folliculogenesis, abnormal expression patterns of target genes, CDKN2A overexpression and enhanced ROS levels in BPA-treated rats. This study provides insights on the effects of BPA exposure on ovulatory, hormonal, mitochondrial dysfunction, and senescence that benefit in better understanding of PCOS induced by BPA.
科研通智能强力驱动
Strongly Powered by AbleSci AI