2D Co metal‐organic framework nanosheet as an oxidase‐like nanozyme for sensitive biomolecule monitoring

Nanozyme‐based biomolecules sensitive and quantitative detection is an attractive strategy due to their high chemical, thermal stability and reactive activity. Here, we have synthesized a significant number of two‐dimensional (2D) cobalt‐metal‐organic framework (Co‐MOF) nanosheets with oxidase (OXD)‐like activity using a facile solvothermal method in one pot for biomolecule monitoring. The synthesized Co‐MOF nanosheets exhibit strong stability, higher specific surface area and more active sites due to their MOF structure. Such Co‐MOF nanosheets with excellent OXD‐like activity show adequate analytical performance in the quantitative determination of dopamine (DA) and glutathione (GSH) with a wider dynamic sensing range and lower detection limits (DA and GSH: 0.24 and 0.067 μmol·L −1 , 3 σ /slope, where σ is standard deviation of the blank). This work extends the application of 2D‐MOF structures in bioassays and brings new insights into the application of OXD‐like nanozymes in bioassays.