Chemical constituents from ethyl acetate extract of Cynanchum otophyllum Schneid.

Fourteen compounds were isolated and purified from ethyl acetate extract of Cynanchum otophyllum Schneid.,and were identified as otophylloside A(1),otophylloside B(2),2,5-dihydroxyacetophenone(3),methyl 2,5-dihydroxy-benzoate(4),vanillin(5),arjunolic acid(6),glycosmisic acid(7),caudatin-3-O-β-D-cymaropyranosyl-(1→4)-β-D-oleandropyranosl-(1→4)-β-D-cymaropyranosyl-(1→4)-β-D-cymaropyranoside(8),penupogenin(9),caudatin-3-O-β-D-glucopyranosyl-(1 →4)-β-D-oleandropyranosyl-(1→4)-β-D-cymaropyranosyl-(1→4)-β-D-cymaropyranoside(10),qingyangshengenin-3-O-β-D-oleandropyranosyl-(1→4)-β-D-cymaropyranosyl-(1→4)-β-D-digitoxopyranoside(11),and kidjoranin-3-O-β-D-cymaropyranoside(12),qingyangshengenin-3-O-β-D-cymaropyranosyl-(1→4)-β-D-digitoxopyranoside(13),and kidjoranin-3-O-β-D-oleandropyranosyl-(1→4)-β-D-cymaropyranosyl-(1→4)-β-D-cymaropyranoside(14) by physicochemical properties and spectroscopic analyses.Among them,compounds 6-7 were firstly isolated from the genus Cynanchum,and compounds 3-5,8-14 were isolated from this plant for the first time.