Antimicrobial activity is one of the most important biological activities of propolis. Propolis extracts from different extraction methods all have significant antimicrobial activities, including antibacterial,antifungal, antiparasitic and antiviral activity. In this article, we presented a review on the related research progress on antimicrobial activity of various propolis extracts for the last decade, aiming to provide references for further research and development and utilization of its antimicrobial activity.