Adjuvant effect of aluminum hydroxide nanoparticles on Newcastle disease antigen in chickens

To evaluate the adjuvant effect and mechanism of aluminum hydroxide nanoparticles,three-week-old commercial chickens were immunized with aluminum hydroxide nanoparticles and conventional aluminum hydroxide adjuvant inactivated vaccine respectively.The hemagglutination inhibition antibody titer,CD4 and CD8 mRNA from peripheral blood lymphocytes on days 2 to 24 after immunization were compared.Aluminum hydroxide nanoparticles adjuvant and conventional aluminum adjuvant induced antibodies with peak titer were 6.67±0.58 and 4.33±0.58 on day 10 post-immunization,and the effective protective immune period was continuous for more than 9 days and less than 7 days,respectively.Compared with the conventional aluminum adjuvant,the aluminium hydroxide nanoparticles adjuvant induced to produce much higher CD4 and CD8 mRNA on days 4 to 17 and 8 to 24 after immunization,respectively.The results demonstrated that the aluminium hydroxide nanoparticles adjuvant could initiate humoral immune and cellular immune response strongly than the conventional aluminum adjuvant.