The activities of main detoxifying enzymes including carboxylesterase,acetylcholinesterase,glutathione S-transferase in Bemisia tabaci pseudopupas on CrylAc+CpTI contton SGK321 and its parent SY321 were determined.The results indicated that SGK321 had no significant effect on main detoxifying enzymes activities in Bemisia tabaci pseudopupas.However,the individual distribution frequency of these detoxification enzymes was different between the two populations.Compared to SY321,α-NA CarE frequency of SGK321 was higher at 30 mOD/(mg protein·min),but lower at 30~40 mOD/(mg protein·min);the acetylcholinesterase frequency of SGK321 was lower at 2~4 U/mg protein while increased 10 percentage points at 4 U/mg protein;the glutathione S-transferase frequency of SGK321 decreased slightly at 80~140 U/mg protein,but increased 15 percentage points at 140~170 U/mg protein.