Expression and localization of LAT1 and 4F2hc in lactation mammary gland of dairy cow

In this research,the expression and localization of LAT1 and 4F2hc in lactation dairy cow mammary gland and in dry period mammary gland were detected by qRT-PCR and immunofluorescence.The results showed that in lactation mammary gland with high protein,LAT1 was located in the basement membrane of mammary epithelial cells.4F2hc was expressed in the cytoplasm of mammary epithelial cells.The expression of LAT1 and 4F2hc in lactation mammary gland with high protein were higher than those in lactation mammary gland with low protein and dry period mammary gland.It indicated that LAT1and 4F2hc may play an important role to regulate milk protein synthesis.