Treatment strategy for difficult blood cross matching caused by autoantibody

Objective:To understand autoantibody status by the serological analysis for difficult blood cross matching caused by autoantibody,and explore the test strategy for positive autoantibody patients.Method:Using saline method(room temperature,37℃,4℃),Polybrene method,Traditional antihumanglobulin test,microcolumn gel immuno-assay identified the antibody specificity for autoantibody patients.According to the characteristics of autoantibodies in patients,different experimental methods were used for blood cross matching in order to choose the right blood for clinical,and the effect of transfusion was observed.Result:In 119positive autoantibodies patients,88samples were warm autoantibodies(73.95%).Among them,12patients were with specific antibodies:Anti-cE 7,anti-E 2,anti-Jkb 1,anti-Lea1,anti-Leb 1.And 9patients were with specific anti autoantibodies:anti-Ce3,anti-E 2,anti-c 1,anti-cE 1,anti-M 1cases.Twenty samples were cold autoantibodies(16.81%),including 1cases with anti-E antibody.Four samples were warm and cold mixed autoantibodies(3.36%);7samples were hyperglobulinemia(5.88%).According to the characteristics of autoantibodies,using different treatment measures achieved satisfactory effect of transfusion.Conclusion:In patients with positive autoantibodies,different autoantibodies characteristic for blood test would be different.In order to ensure the clinical blood transfusion safety,reasonable and effective,we should master the indications for transfusion,to avoid unnecessary blood transfusion.For blood cross match test,we should select suitable experimental methods according to the antibody characteristic,to eliminate the autoantibodies effect in patients.