Isolation and identification of lactobacillus from female genital tract

Objective:Isolating lactobacillus from health female genital tract, for exploring probiotics to research compound preparation.Methods:Traditional morphology analysis was used to identify lactobacillus, and further screen hydrogen peroxide-producing lactobacillus.The molecular biology method was used to identify exactly and to examine predominant strain by performance test.Results:There were 11 strains of lactobacillus isolated and identified from 18 health examination women genital tract, the detection rate was 61.1%.According to the results of these experiment,11 strains were identified that 4 were lactobacillus acidophilus, 3 were lactobacillus gasseri, 2 were lactobacillus jensenii,1 was lactobacillus crispatus and another one was lactobacillus salivarius.Conclusion:Traditional biochemical identification and molecular biology methods could identify lactobacillus.Only lactobacillus crispatus is hydrogen peroxide-producing one. After the preliminary test, the strain is a strong acid-resistant and hot-resistant one, and is bacteriostasis in vitro testing.